Preparation of CSPD Chemiluminescent Substrate Solution

The CSPD Chemiluminescent substrate is provided in the Great EscAPe kit as a 25 mM stock solution. This solution should be stored in the dark at 4°C, and is stable in this form for 6 mo. A final CSPD concentration of 1.25 mM is recommended for chemiluminescent detection of SEAP activity (20X dilution of CSPD stock solution). The stock CSPD is diluted with a chemiluminescent enhancer solution provided as a component of the Great EscAPe kit. The dilution with enhancer should be performed just prior to use. Keep the diluted substrate solution on ice while performing the assay.

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