Transfection and Analysis of hGH Expression

1. 2X HBS. 16.4 g NaCl, 11.9 g HEPES, 0.21 g Na2HP04, add dH20 to 1 L, adjust pH to 7 05 with NaOH, and sterilize through a 0 45-pm filter

2 0 25MCaCl2 (sterile)

3 Calf thymus DNA (Sigma) dissolved in TE at concentration of 2 mg/mL

4 CsCl-prep grade DNA.

5 Glycerol

6 12 x 75 mm sterile disposable polystyrene tube (Fisher, Pittsburgh, PA)

7 Nichols Institute hGH Assay Kit (#40-2155)

8 Rotating platform 9. y-radiation counter

10 Spreadsheet program such as Microsoft Excel or Lotus 1-2-3

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