Gal Plates

Start with the basic recipe for dropout plates, but mix basic ingredients in 900 mL of dH20 rather than 1 L. Autoclave 20 min, and allow to cool to ~65°C; then add 100 mL of sterile filtered 10X BU salts (1 L 10X stock = 70 g Na2HP04 • 7H20, 30 g NaH2P04, pH adjusted to 7.0, filter sterilized), and 2 mL of a 40 mg/mL solution of X-gal dissolved in dimethylformamide (DMF). After pouring, plates should be stored at 4°C and protected from light

X-Gal plates required for the experiments described below ura-, made with glucose; ura-, made with 2% galactose + 1% raffmose.

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