Gabab1gaba

Fig. 3. Distribution of GABAB(1) and GABAB(2) mRNA by in situ hybridization using variant non-specific oligonucleotides (a), (b), GABAB(1) and GABAB(2) proteins by immunohistochemistry using variant non-specific antibodies (c), (d) and GABAb binding sites by receptor autoradiography (e). The mismatch of mRNA signals in the striatum is clear in (a) and (b). A comprehensive overlap of GABAB(1) and GABAB(2) protein localization is evident from immunohistochemistry with polyclonal antibodies (c) and (d) with cortex, hippocampus thalamus and cerebellum showing high levels of receptor subunit expression. Part (e) shows the binding of 0.5 nm [3H]-CGP62349, a high-affinity antagonist radioligand for GABAb receptors, which is in good agreement with immunohistochemical localization (c) and (d) [(e) image courtesy of Professor N. Bowery, University of Birmingham, UK]. Labels: 1, striatum; 2, brainstem; 3, thalamus; 4, hippocampus; 5, cerebellum; 6, cortex. Scale bar, 1mm.(Adapted from Billinton et al., 2001, Copyright Elsevier with permission.)

Fig. 3. Distribution of GABAB(1) and GABAB(2) mRNA by in situ hybridization using variant non-specific oligonucleotides (a), (b), GABAB(1) and GABAB(2) proteins by immunohistochemistry using variant non-specific antibodies (c), (d) and GABAb binding sites by receptor autoradiography (e). The mismatch of mRNA signals in the striatum is clear in (a) and (b). A comprehensive overlap of GABAB(1) and GABAB(2) protein localization is evident from immunohistochemistry with polyclonal antibodies (c) and (d) with cortex, hippocampus thalamus and cerebellum showing high levels of receptor subunit expression. Part (e) shows the binding of 0.5 nm [3H]-CGP62349, a high-affinity antagonist radioligand for GABAb receptors, which is in good agreement with immunohistochemical localization (c) and (d) [(e) image courtesy of Professor N. Bowery, University of Birmingham, UK]. Labels: 1, striatum; 2, brainstem; 3, thalamus; 4, hippocampus; 5, cerebellum; 6, cortex. Scale bar, 1mm.(Adapted from Billinton et al., 2001, Copyright Elsevier with permission.)

ganglia (Fig. 4). Although the interaction between the GABAa receptor subunit and GABAB(1) did not form a functional receptor, the g2S subunit association resulted in an enhancement of the normal GABAB heterodimeric receptors' response to GABA (Balasubramanian et al., 2004). Given the close association between the GABAA and GABAB receptors in the basal ganglia (Fig. 4) this may enhance the efficacy of GABA at GABAB receptors.

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