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aThe Tm measurements were carried out in 150 mM Na cacodylate buffer (140 mM NaCl added to 10 mM Na cacodylate buffer). The reproducibility of the Tm values was ± 1°C for three or four measurements.

fcATm = Tm(1 mM) - Tm(0), where Tm(1 mM) is the Tm of the various hybrids in the presence of 1 mM polyamine and Tmp) is the Tm of the hybrid in 150 mM Na cacodylate buffer, in the absence of polyamine. Reprinted with permission from ref. 12.

m aThe Tm measurements were carried out in 150 mM Na cacodylate buffer (140 mM NaCl added to 10 mM Na cacodylate buffer). The reproducibility of the Tm values was ± 1°C for three or four measurements.

fcATm = Tm(1 mM) - Tm(0), where Tm(1 mM) is the Tm of the various hybrids in the presence of 1 mM polyamine and Tmp) is the Tm of the hybrid in 150 mM Na cacodylate buffer, in the absence of polyamine. Reprinted with permission from ref. 12.

Dna Major Groove
Fig. 11. Representation of a triplex forming oligonucleotide binding in the major groove of DNA. (Reprinted from ref. 46 with permission from Oxford University Press.)

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