Theories of PolyamineDNA Binding

The exact mechanism of polyamine binding to DNA is a contentious issue. Some researchers believe that polyamine-DNA interaction is primarily electrostatic in

Fig. 19. Precipitation/resolubilization of sonicated (A) and native (B) calf thymus DNA by spermine in the presence of 10 (•), 25 (□), 50 (▲), 100 (▼), 150 (♦), and 250 (o) mMNa+. A logarithmic scale is used for spermine concentrations. Experiments were performed in 10 mM cacodylate buffer (pH 7.4). Higher concentrations of Na+ were achieved by adding small volumes of 4 M NaCl solution. (C) Concentrations of spermine required for 50% DNA precipitation (open symbols) and resolubilization (filled symbols) of sonicated (o,^) and native (□,■) calf thymus DNA. Logarithmic scale is used for both xandyaxes of (C). (Reprinted with permission from ref. 62. © 1999 American Chemical Society.)

Fig. 19. Precipitation/resolubilization of sonicated (A) and native (B) calf thymus DNA by spermine in the presence of 10 (•), 25 (□), 50 (▲), 100 (▼), 150 (♦), and 250 (o) mMNa+. A logarithmic scale is used for spermine concentrations. Experiments were performed in 10 mM cacodylate buffer (pH 7.4). Higher concentrations of Na+ were achieved by adding small volumes of 4 M NaCl solution. (C) Concentrations of spermine required for 50% DNA precipitation (open symbols) and resolubilization (filled symbols) of sonicated (o,^) and native (□,■) calf thymus DNA. Logarithmic scale is used for both xandyaxes of (C). (Reprinted with permission from ref. 62. © 1999 American Chemical Society.)

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