Triplex DNA Stabilization

Triplex DNA has assumed much attention during the past three decades because of the potential gene regulatory effects of triple helix-forming oligonucleotides, and subsequently for their use as therapeutic agents (46). Triplex forming poly(purine)/poly

Fig. 10. Melting profile of the PO-DNA-RNA, PS-DNA-RNA, PO-DNA-PO-DNA, PS-DNA-PS-DNA, PS-DNA-PO-DNA, and RNA-RNA helices. The concentrations of spermine used were 0 (•), 1 (O), 5 (▼), 10 (v), 25 (□), 50 (■), and 100 ^M(♦). The melting temperature measurements were carried out in 10 mM cacodylate buffer at a heating rate of 0.5°C/min for all the hybrid helices. (Reprinted with permission from ref. 12. © 2005 American Chemical Society.)

Fig. 10. Melting profile of the PO-DNA-RNA, PS-DNA-RNA, PO-DNA-PO-DNA, PS-DNA-PS-DNA, PS-DNA-PO-DNA, and RNA-RNA helices. The concentrations of spermine used were 0 (•), 1 (O), 5 (▼), 10 (v), 25 (□), 50 (■), and 100 ^M(♦). The melting temperature measurements were carried out in 10 mM cacodylate buffer at a heating rate of 0.5°C/min for all the hybrid helices. (Reprinted with permission from ref. 12. © 2005 American Chemical Society.)

(pyrimidine) sequences are found in the promoter elements of several genes, including c-myc, HER-2, and Ets-2. A ribbon model representation of triplex DNA is shown in Fig. 11. Triple helix formation involves the binding of a short oligonucleotide via hydrogen bonding to the major groove of a double helix. The widely accepted structural model for polypurine/(polypyrimidine)2 triple helix is based on X-ray fiber diffraction studies. In this structural model, an A-form polypurine/polypyrimidine duplex

Table 2

Effects of Polyamines on the Stability of Various Hybrids in the Presence of 150 mM Na+

Table 2

Effects of Polyamines on the Stability of Various Hybrids in the Presence of 150 mM Na+

Concentration polyamines (mM)

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