Heart Failure A Genomics Approach

Heart failure is a syndrome that occurs over time as the heart becomes less and less able to pump blood at a rate that is sufficient to meet the requirements of the body's organs, tissues and cells. For example, cardiomyopathies, which are disorders affecting the heart muscle, are a predominant cause of heart failure 1 . Heart failure may be right sided, left-sided, systolic or diastolic, or both. Clinically, systolic heart failure, an impairment of the right ventricle's ability to eject...

Monocytes Macrophages

Monocytes play a pivotal role in various human infectious and inflammatory diseases and participate as a key effector in the formation of the atherosclerotic plaque. SAGE analysis in lipopolysaccharide (LPS)-stimulated human monocytes 39 yielded total of 35,874 tags corresponding to more than 12,000 different transcripts. LPS-inducible gene products are involved in cell activation and migration, angiogenesis, tissue remodeling, metabolism, and inflammatory reactions (including IL-6, IL-1a, IL-1...

Designing Meaningful Experiments

The number of review articles on gene expression technologies probably exceeds the number of primary research publications in this field 13 . This is not the result of any paucity of primary data since Microarray Core Facilities have been established in nearly every academic institution, each processing hundreds of micro-arrays each year, and numerous laboratories manufacture and run their own cDNA microarrays, resulting in millions of independent gene expression measurements. One might argue...

CDNA Microarrays or Oligonucleotide Arrays

The construction of gene expression databases requires technologies that can accurately and reproducibly measure changes in global mRNA expression levels. Ideally, these technologies should be able to screen all gene transcripts, be applicable across a wide range of cell and tissue types, require minimal amounts of biological material, and be capable of processing large number of samples. Although several different technology platforms have been developed 4-9 , each comes with its own set of...

List of Contributors

Ruedi Aebersold Institute for Systems Biology 1441 North 34th Street Seattle, WA 98103-8904 USA E-mail raebersold systemsbiology.org http www.systemsbiology.org Alfred C. Aplin Department of Pathology University of Washington Seattle, WA 98195-7335 USA Richard Lewar Center of Excellence EN 12-324 Toronto General Research Institute 200 Elizabeth Street Toronto, ON M5G 2C4 Canada Aab Institute of Biomedical Sciences Roger E. Bumgarner Departments of Microbiology University of Washington Seattle,...