Modeling of Interactions on IMAC

The SMA model of IEX was extended to IMAC systems [77] since this mode of chromatography is also based upon interactions of the protein with discrete binding sites on the surface and imidazole acts analogous to salt ions in IEX by binding to a single chelating site on IMAC. The key difference lies in the relatively higher affinity of imidazole for the chelation sites. Upon interaction, the protein interacts with nP sites on the stationary phase and shields aP metal ion sites.

1 + Km Cm where the subscripts P and m refer to the protein and the mobile-phase modulator, respectively, nP is the number of interaction sites of the protein with the surface, L is the bed capacity determined by imidazole binding alone, and K is the equilibrium constant.

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