Haemoglobin electrophoresis

HbS can be identified by cellulose acetate electrophoresis at pH 8.4 (Table 7.1 and Figure 7.5b). HbD and HbG have the same electrophoretic mobility with this method, but can be distinguished using citrate agar electrophoresis at pH 6.2 or thin-layer isoelectric focusing. Distinction cannot be made between HbSS and HbS P0-thalassaemia on electrophoresis. The diagnosis of

Hbas Hbaa And Hbsc Electrophoresis

Figure 7.5 Peripheral blood smear from an individual with sickle cell anaemia (a). Haemoglobin electrophoresis (b), showing astandard, b- and c-HbSS, d-HbSC, e-sickle trait. Sickle solubility assay (c) is positive (tube a) in all three conditions.

Figure 7.5 Peripheral blood smear from an individual with sickle cell anaemia (a). Haemoglobin electrophoresis (b), showing astandard, b- and c-HbSS, d-HbSC, e-sickle trait. Sickle solubility assay (c) is positive (tube a) in all three conditions.

S-P°-thalassaemia is suggested by microcytosis and elevated HbA2, and confirmed by finding P-thalassaemia trait in one of the parents. HbA and HbS are observed upon electrophoresis in both sickle cell trait and HbS P+-thalassaemia; however, the HbA fraction is greater than 5°% in the former, but ranges from 5% to 3°% in the latter. The level of HbF is variably elevated with higher levels observed in patients with the Arab-Indian and Senegal haplotypes.

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