Technical Considerations

Anti-vimentin antibodies are among the most versatile of all commercially available antibodies. The vimentin intermediate filaments are able to survive routine tissue processing and require no specific techniques to expose the antigens. However, pretreatment with pro-

teinase K augments the sensitivity of anti-vimentin antibodies. HIER also augments staining intensity (16). Strong cytoplasmic staining can be identified within mesenchymal cells in the normal skin. This quality can be used as an appropriate positive internal control. In normal conditions, keratinocytes do not express vimentin, allowing for a good internal negative control.

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