Technical Considerations

Anti-vimentin antibodies are among the most versatile of all commercially available antibodies. The vimentin intermediate filaments are able to survive routine tissue processing and require no specific techniques to expose the antigens. However, pretreatment with pro-

teinase K augments the sensitivity of anti-vimentin antibodies. HIER also augments staining intensity (16). Strong cytoplasmic staining can be identified within mesenchymal cells in the normal skin. This quality can be used as an appropriate positive internal control. In normal conditions, keratinocytes do not express vimentin, allowing for a good internal negative control.

Think Clean and Green to Flawless Skin

Think Clean and Green to Flawless Skin

Lets accept the fact: many of us are skin conscious. As much as possible, we wanted to have a fresh, good looking skin. However, many of us failed to recognize that simple steps are the best ways to attain it.

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