1. Complete factorial 2 x 3 x 3 design. To perform the growth and bacteriocin-production experiments for L. salivarius CRL 1328, a design of three factors with 2, 3, and 3 levels, respectively, is used. This design has in 18 different treatments total (2 x 3 x 3).
2. Fractionated factorial design. The screening of growth curves and H2O2 production by L. crispatus CRL 1266 and L. paracasei CRL 1289 is performed by using a factorial fractional 25-1 design, with eight central points. A total of 24 different combinations are tested. The fractionated factorial design was obtained by using the MINITAB software.
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