Flow Cytometric Analysis

1. Determine analysis parameter settings for determination of viability of cells.

2. Use control suspensions (heat killed and viable) to select analysis parameters on the flow cytometer. Heat-killed cells (90°C for 20 min) stained with PI (final concentration 25 mg/L) fluoresce red with a 585/42 bandpass filter. Viable cells exclude PI and are detected with a 530/30 bandpass filter. Sample parameter settings are given in Table 1.

Table 1

Sample Parameter Settings of Flow Cytometric Analysis

Detector Compensation

Detector Compensation

Table 1

Sample Parameter Settings of Flow Cytometric Analysis

Settings

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