Growth Media

1. Growth medium for Enterococcus sp.: LAPTg broth: 15g/L Peptone, 10g/L Tryptone, 10g/L yeast extract, 10g/L glucose, and 1 mL/L Tween-80, final pH 6.5. Sterilize at 121°C for 20 min.

2. Growth medium for Listeria sp.: BHI (brain-heart infusion, BioKar Diagnostics), final pH 6.5. Sterilize at 121°C for 20 min (see Note 1).

3. Growth medium for Lactobacillus sp.: de Man-Rogosa-Sharpe (MRS) broth (BioKar Diagnostics), final pH 6.5. Sterilize at 121°C for 20 min.

4. Medium for bacteriocin activity assays: BHI base agar (1.5% agar) and BHI top agar (0.7% agar).

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