Info

Separating gel

Compacting gel

Reactive

(12% of Polyacrylamide)

(4% of Polyacrylamide)

Bidistilled water

1.658 mL

1.525 mL

Solution B: 1.5 M Tris-HCl, pH 8.8

1.25 mL

Solution C: 0.5 M Tris-HCl, pH 6.8

0.625 mL

Solution D: 10% SDS

50 ^L

25 ^L

Solution A: acrylamide/bis

2 mL

0.325 mL

(30% T/27% C)

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