Info

10% APS (0.05%)

37 ^L

27.5 ^L

TEMED (0.05%)

2.5 ^L

2.5 ^L

APS, ammonium persulfate; SDS, sodium dodecyl sulfate; TEMED, tetramethylethylenediamine.

APS, ammonium persulfate; SDS, sodium dodecyl sulfate; TEMED, tetramethylethylenediamine.

6. To ensure that recovered DNA belonged exclusively to bacteriophages and was not free DNA, the phage suspension was treated with 10 U of DNase and RNase per mL for 30 min as previously described (12).

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