1. Recovery index of infectant larvae (L3). This index is considered to be the relationship between the number of L3 larvae obtained, and the egg counts by gram of feces (eggs per gram [epg]) multiplied by 3. Significant differences between the recovery indices obtained from control and test coprocultures are calculated, to establish the possible action of probiotic bacteria on parasitic eggs or first larval stages.
2. Dead L3 larvae number. Compare control and test coprocultures for dead L3 larvae numbers. A statistically significant difference between them indicates a larvicidal action from the bacteria studied (see Note 5).
1. Make notes of (1) the date of sample collection; (2) the time (mainly if the sample doesn't take chemical preserving because some helminthic eggs evolve quickly, which can make later identification difficult); (3) the animal species; (4) identification of the animal (for individual samples) or number of animals (for a flock); (5) sample consistency; (6) presence of mature parasites or blood, or any other fact that you consider important to remember.
2. This procedure allows separation of cysts, eggs, and other parasitic structures by employing a liquid of a higher specific weight that those of the parasites. Then parasites are recovered from the surface, and organic matter remains at the bottom of the recipient.
3. NaCl-saturated solution is the most utilized, but others hypersatured solutions such as magnesium sulphate (density 1.3 g/L), or zinc sulfate (density 1.2-1.25 g/L) can be used.
4. The fecal samples collected for coprocultures do not contain preserving solutions. The use of preserving solutions is only for to obtaining from fecal eggs different larval nematodes that are so similar they cannot be identified. On the other hand, observation of larvae obtained, allows systematic classification of different nematode parasites.
5. All laboratory material must be washed with water and hypochlorite sodium after use.
6. If it is not possible to identify all the larvae the same day, the assay tube can be maintained under refrigeration (12-14°C) for up to 4 mo.
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