Materials

1. Nycodenz (N,N'-bis[2,3 dihydroxypropyl]acetamido-2,4,6-tri-iodo-isophthalamide; Histodenz™, Sigma, St. Louis, MO).

2. Centrifuge tubes, ultraclear, for swing bucket rotor.

3. Immunomagnetic separation kit (Crypto-Scan®IMS, ImmuCell, Portland, ME) or Dynabeads anti-Cryptosporidium (Dynal, Lake Success, NY), or Aureon Crypto Kit, (Aureon Biosystems, Vienna, Austria).

4. Ethyl ether.

5. STE buffer: 100 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA.

6. Geneclean kit for DNA isolation (Qbiogene, Carlsbad, CA).

7. Zirconia/silica or glass beads, 0.5 mm (Biospec Products, Bartlesville, OK).

8. Mini-Beadbeater (Biospec Products).

9. 10X PCR buffer: 40 mM Tris-HCl, pH 8.3, 200 mM KCl, 0.004% gelatin.

10. 100 mM Deoxynucleoside triphosphates (Promega, Madison, WI).

11. 25 mM MgCl2 solution.

12. Thermal cycler (MJ Research, Watertown, MA).

13. LightCycler (Roche, Diagnostics, Mannheim, Germany).

14. Polynucleotide kinase.

16. TBE buffer: 0.09 M Tris-borate, 1 mM EDTA, pH 8.0.

17. Spreadex Polymer NAB (Elchrom Scientific, Cham, Switzerland).

18. High Pure PCR template preparation kit (Roche Diagnostics).

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