Nematodes

2.2.1. Collection of Fecal Samples

1. Latex gloves.

2. Very clean glass or plastic recipients (sterility is not necessary), with covers, to be labeled.

3. Recipient with ice for transporting the samples under refrigeration (at 4°C).

2.2.2. Microscopic Examination of Previous Enrichment

1. Fecal samples.

2. 25 mL Precipitation glasses.

3. Glass rods.

4. Strainer with wire mesh.

5. Cover slips and slides.

6. Lugol solution.

7. NaCl saturated solution.

2.2.3. Fecal Nematode Egg Counts

1. Fecal samples.

2. McMaster recount camera.

3. Mortar with pestle (for pounding).

4. Strainer with wire mesh.

5. Pasteur pipets.

6. 100 mL NaCl-saturated solution.

2.2.4. Coprocultures

1. Fecal samples.

3. Mortar with pestle (for pounding).

4. Pasteur pipets.

5. Distilled or mineral water (without chlorine).

6. Inert material (5-mm Telgopor pearls).

7. 1-mL Bacterial suspensions.

8. Assay tubes.

2.2.5. Third-Stage Larvae (L3) Counts

1. Camera to count the larvae.

2. Pasteur pipets.

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