Contents

Dedication v

Preface vii

Acknowledgments ix

Contributors xiii

List of Color Pages xv

Part I. Basic Methodology

1. New Rapid Multicolor PRINS Protocol 3

Ju Yan, Macoura Gadji, Kada Krabchi, and Régen Drouin

2. PRINS Combined With Peptide Nucleic Acid Labeling 1 5

Franck Pellestor and Petra Paulasova

3. PRINS Combined With Indirect Immunofluorescence 23

Philippe Coullin

4. PRINS for the Detection of Unique Sequences 33

Stephen S. Wachtel and Avirachan T. Tharapel

5. Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS) 41

J0rn Koch

6. Analysis of Sperm Aneuploidy by PRINS 49

Franck Pellestor, Brigitte Andréo, Jacques Puechberty,

Geneviève Lefort, and Pierre Sarda

7. In Situ PCR Protocols 61

Omar Bagasra and Twaina Harris

Part II. Research and Clinical Applications

8. Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS 81

J0rn Koch

9. PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei 89

Antonella Russo

10. PRINS for the Detection of Gene Deletions in Cancer 1 05

Avirachan T. Tharapel and Stephen S. Wachtel

11. Combined Fluorescence In Situ Hybridization and PRINS

for the Analysis of the Dystrophin Gene 11 5

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