Detection and Microscopy

1. Drain the excess washing solution off the slide.

2. Mount the slide in Vectashield antifade solution containing a mix of propidium iodide (0.3 pL/mL) and DAPI (0.3 pL/mL). Use 15 to 20 pL of mountant/slide.

3. Cover with a 22 x 40 cover slip and seal the cover slip with rubber cement.

4. Examine the slide under the epifluorescence microscope equipped with suitable filters. Preferentially, use first the triple band-pass filter and confirm the coloring of the fluorescent spot with single band-pass filters. Figure 1 shows typical results obtained on a human metaphase.

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