Detection and Microscopy

1. 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI).

2. Propidium iodide (Sigma).

3. Antifade solution Vectashield (Vector Labs, Burlingame, CA).

5. Rubber cement (Artos).

6. Epifluorescence Microscope Leica DMRB (Leica, France) equipped with x40 and x100 Plan FluoTar objectives and with a DAPI single band-pass filter (Leitz filter A, cat. no. 513804), an FITC single band-pass filter (filter I3, cat. no. 513808), a tetramethylrhodamine isothiocyanate (TRITC) single band-pass filter (filter N2.1, cat. no. 513812), an FITC/TRITC double band-pass filter (filter G/R, cat. no. 513803), and a triple filter (filter B/G/R, cat. no. 513836) for simultaneous observation of DAPI/Cascade-Blue, FITC, and TRITC signals.

7. For image capturing, we use the software Metasystem Isis, Version 5.0 (Metasystem, Altlusshein, Germany).

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