Before one can perform in situ polymerase chain reactions (PCRs) using the protocols described herein, utmost care first should be taken to preserve the DNA and mRNA molecules inside the cellular structures. Therefore, we recommend using ultraclean glass slides. The slides can be purchased from any reliable source. Furthermore, it would be wise to autoclave or sterilize the slides (also discussed herein) before applying cells or tissue sections to them. We realize that in many circumstances this may not be possible because often imes a bench scientist will receive tissues slides already mounted in a clinical laboratory elsewhere.
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