Omar Bagasra and Twaina Harris

Summary

This chapter provides detailed methods and material descriptions of in situ polymerase chain reaction protocols. It describes all the essential components of the technique, various protocols suitable for different kinds of tissues and cell preparations, and also gives various instructions for troubleshooting. We have declined to devote detailed discussions on in situ hybridization because there are several excellent articles and books available on this subject.

Key Words: In situ hybridization; PCR; RT-PCR; probes; primers; amplification; chambers.

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