Slide Preparation

1. Check the slides under the light microscope to ensure that both the cell concentration and the spreading are optimum.

2. Dehydrate the slides by passage through an ethanol series (70%, 90%, 100%) at room temperature, 3 min for each step, and air dry.

3. Denature chromosomal DNA by immersing the slides in 70% formamide, 2X SSC, pH 7.0, at 73°C for 4 min (see Note 4).

4. Pass slides through an ice-cold ethanol series (70%, 90%, 100%), 3 min for each step, and air dry.

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