Slide Preparation

1. IX phosphate buffer saline (PBS): 1/10 solution in distilled water from PBS 10X (Gibco BRL; see Note 2).

2. Cytospin: cytospin 2 (Shandon, Eragny, France).

3. Fixative: 90% methanol/10% acetic acid (Carlo Erba, Rodano, Italy; see Note 3).

4. Cell suspension medium: 1X PBS, 0.1% ethylene diamine tetraacetic acid, 0.2% BSA.

5. Permeabilization medium: 1X PBS, 1% Triton X-100 (Sigma).

6. Denaturation solution: 70% formamide, 30% 2X standard saline citrate (SSC), pH 7.0-7.2 (see Note 4).

7. 20X SSC: 3MNaCl, 0.3 sodium citrate, pH 7.4 (Gibco, BRL).

8. 2X SSC: 1/10 20X SSC buffer in distilled water (see Note 2).

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