D

Fig. 9. Kinetics of the formation of Cys-DNIC in HEPES buffer (100 mM, pH = 7.4) as detected by EPR. (A) Mixture of 20 mM cysteine, 400 ^M Cys-NO, 100 ^M Fe2+ and 500 ^M sodium citrate. (B) Mixture of 20 mM cysteine, 200 ^M NO-proline, 100 ^M Fe2+ and 500 ^M sodium citrate. (C) Mixture of 10 mM cysteine, 400 ^M Cys-NO, 50 ^M Fe2+ and 250 ^M sodium citrate and (D) Complete EPR spectrum of the solution (A). (From Ref. [19].)

600 1000 4500 2000 2500 time, s

Fig. 9. Kinetics of the formation of Cys-DNIC in HEPES buffer (100 mM, pH = 7.4) as detected by EPR. (A) Mixture of 20 mM cysteine, 400 ^M Cys-NO, 100 ^M Fe2+ and 500 ^M sodium citrate. (B) Mixture of 20 mM cysteine, 200 ^M NO-proline, 100 ^M Fe2+ and 500 ^M sodium citrate. (C) Mixture of 10 mM cysteine, 400 ^M Cys-NO, 50 ^M Fe2+ and 250 ^M sodium citrate and (D) Complete EPR spectrum of the solution (A). (From Ref. [19].)

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