If cysteine decomposes the protein-bound DNIC, it is expected that "free" iron will be released from the complex. To monitor the iron release, the iron indicator a, a-dipyridyl was added to the samples during incubation of the ferredoxin DNIC with cysteine [55]. Very little iron was released from the ferredoxin DNIC during incubation with a, a-dipyridyl alone, but with the inclusion of L-cysteine, most of the iron originally contained in the ferredoxin was released (Fig. 3).

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