Rsno As No Donors

Scheme 1.

DNIC can influence a number of biochemical and physiological events acting as NO and NO+ (RS—NO) donors. The powerful biological activity of these complexes was discovered in the 1980s by using synthetic DNIC with both thiol-containing and non-thiolic ligands. The experiments demonstrated high hypotensive [15-17] and anti-aggregative capacity of the complexes [18,19]. Later, the high vasodilatory activity of the DNIC was also found [20,21]. Now a great body of data on these properties of DNIC has been accumulated and is considered in this chapter.

HYPOTENSIVE ACTIVITY OF DNICs

The realization that DNICs have important biological potential began after the finding that a range of low-molecular DNICs (DNIC with cysteine, thiosulfate or phosphate) possesses long-lasting hypotensive activity after a bolus injection of the complexes into the portal vein of rats anesthetized with sodium ethaminal [15]. Fig. 1 and Table 1 show the data

Fig. 1. Blood pressure change (ABP, mmHg) in Wistar rats in response to nitroglycerol (NG) 5 nM (1); sodium nitroprusside (SNP) 20 nM (2); or DNIC with thiosulfate 100 nM (2-3 injections) (3); 200 nM DNIC with phosphate (4); DNIC with cysteine 100 nM (5); or DNIC with thiosulfate (2000, 200 and 400 nM, respectively) (single injection) (6-8) [15].

Fig. 1. Blood pressure change (ABP, mmHg) in Wistar rats in response to nitroglycerol (NG) 5 nM (1); sodium nitroprusside (SNP) 20 nM (2); or DNIC with thiosulfate 100 nM (2-3 injections) (3); 200 nM DNIC with phosphate (4); DNIC with cysteine 100 nM (5); or DNIC with thiosulfate (2000, 200 and 400 nM, respectively) (single injection) (6-8) [15].

Table 1 Dose dependence of blood pressure drop (ABP) at initial stage of DNICs, nitroprusside or nitroglycerol (NG) injection to rats [15]

Preparation Dose (10-8 M) ABP (mmHg)

Nitroprusside

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