Fig. 12. Formation of the DNIC-phosphate complex as detected by EPR at room temperature in HEPES. (A) Kinetics of the DNIC-phosphate complex in a solution with 100 mM phosphate, 400 ^M Cys-NO, 50 mM Fe2+ and 250 ^M sodium citrate. (B) Idem with 20 mM cysteine. (C) 100 mM phosphate, 400 ^M NO-proline, 50 m-M Fe2+ and 250 ^M sodium citrate and (D) The EPR signal of the solution (C). (From Ref. [19].)

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