Cultures Of Organised Structures

Organ culture is used as a general term for those types of culture in which an organised form of growth can be continuously maintained. It includes the aseptic isolation from whole plants of such definite structures as leaf primordia, immature flowers and fruits, and their growth in vitro. For the purposes of plant propagation, the most important kinds of organ culture are:

• Meristem cultures, in which are grown very small excised shoot apices, each consisting of the apical meristematic dome with or without one or two leaf primordia. The shoot apex is typically grown to give one single shoot.

• Shoot tip, or shoot cultures, started from excised shoot tips, or buds, larger than the shoot apices employed to establish meristem cultures, having several leaf primordia. These shoot apices are usually cultured in such a way that each produces multiple shoots.

• Node cultures of separate lateral buds, each carried on a small piece of stem tissue; stem pieces carrying either single or multiple nodes may be cultured. Each bud is grown to provide a single shoot.

• Isolated root cultures. The growth of roots, unconnected to shoots: a branched root system may be obtained.

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