Plant tissue culture is the science of growing plant cells, tissues or organs isolated from the mother plant, on artificial media. It includes techniques and methods used to research into many botanical disciplines and has several practical objectives. Before beginning to propagate plants by tissue culture methods, it is necessary to have a clear understanding of the ways in which plant material can be grown and manipulated in 'test tubes'. This chapter therefore describes the techniques that have been developed for the isolation and in vitro culture of plant material, and shows where further information can be obtained. Both organised and unorganised growth are possible in vitro.
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