Stage I Establishing an aseptic culture

The customary second step in the micropropagation process is to obtain an aseptic culture of the selected plant material. Success at this stage firstly requires that explants should be transferred to the cultural environment, free from obvious microbial contaminants; and that this should be followed by some kind of growth (e.g. growth of a shoot tip, or formation of callus ). Usually a batch of explants is transferred to culture at the same time. After a short period of incubation, any container found to have contaminated explants or medium is discarded. Stage I would be regarded as satisfactorily completed if an adequate number of explants had survived without contamination, and was growing on. The objective is reproducibility, not 100% success.

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