Stage II The production of suitable propagules

The object of Stage II is to bring about the production of new plant outgrowths or propagules, which, when separated from the culture are capable of giving rise to complete plants. According to the in vitro procedure that is being followed (Fig 2.1), multiplication can be brought about from newly-derived axillary or adventitious shoots, somatic embryos, or miniature storage or propagative organs. In some micropropagation methods, Stage II will include the prior induction of meristematic centres from which adventitious organs may develop. Some of the propagules produced at Stage II (especially shoots) can also be used as the basis for further cycles of multiplication in that they can usually be cultured again (subcultured) to increase their number.

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