Subculturing Hazards

There are several hazards in subculturing which are discussed more fully in other chapters of this book. Several kinds of callus may arise from the initial explant, each with different morphogenic potential. Strains of callus tissue capable of giving rise to somatic embryos and others without this capability can, for instance, arise simultaneously from the culture of grass and cereal seed embryos. Careful selection of the correct strain is therefore necessary if cultures capable of producing somatic embryos are ultimately required. Timing of the transfer may also be important, because if left alone for some while, non-embryogenic callus may grow from the original explant at the expense of the competent tissue, which will then be obscured or lost.

Although subculturing can often be continued over many months without adverse effects becoming apparent, cultures of most unorganised cells and of some organised structures can accumulate cells that are genetically changed. This may cause the characteristics of the culture to be altered and may mean that some of the plants regenerated from the culture will not be the same as the parent plant. This subject is discussed further in Chapter 2. Cultures may also inexplicably decline in vigour after a number of passages, so that further subculture becomes impossible.

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Growing Soilless

Growing Soilless

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