Bovine Serum Albumin Structures at pH 3 and pH

DPI has been used as a high-resolution (< 0.01 nm), lab-based technique to determine the thickness and refractive index (density) of bovine serum albumin (BSA) molecules adsorbing or reacting at the solid-liquid interface in real time (up to ten measurements per second). Results from these studies of the adsorption of BSA on to a silicon oxynitride chip surface demonstrate how time-dependent molecular behaviour can be examined. Mechanistic and structural information relating to the adsorption process was obtained as a function of the solution pH. The results are shown in Fig. 3, and the schematic representation of the layer changes occurring with pH cycling are shown in Fig. 4.

Fig. 3. Changes in layer structure as a function of cycling pH at a constant bovine serum albumin (BSA) concentration. Light grey thickness, mid grey refractive index (proportional to density), dark grey mass

Fig. 4. Schematic diagram of the structure of the physisorbed BSA layer during the pH changes described for Fig. 3

Fig. 4. Schematic diagram of the structure of the physisorbed BSA layer during the pH changes described for Fig. 3

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