The electrical conducting properties of the carbon felt grafted with redox enzymes with the aid of a polypyrrole polymer layer, allow the electrochemical coupling or electrochemical transformation of the reactants involved in the activity of redox enzymes linked to such immobilization supports. As the enzyme support acts as an electrically conducting membrane, coupling via an electrochemical process is a simple means to intervene in the catalytic enzyme reaction. The electrochemical reaction has been used successfully for the regeneration of a substrate of the enzyme (converting a product of the enzyme activity into substrate) or even for the production of substrate in the vicinity of the enzyme
These two processes have been demonstrated with peroxidase. In a first example the cosubstrate of the peroxidase reaction, for instance Fe(CN)6-, which is oxidized by peroxidase, is reduced electrochemically on the conducting textile biased to a negative electric potential. In the second example, H2O2, a substrate of peroxidase, is electrochemically produced by the reduction of oxygen, applying a reducing electrical potential to the conducting textile (in the latter case the textile acts as a working electrode for oxygen reduction).
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