Enzymatic Membrane Preparation

The enzymatic membrane was prepared in a three-electrodes cell as follows: monomer 1 (2 mM) in 0.1 M NaClO4 acetonitrile, was electropolymerized on a 1.5 X 0.5 X 0.5 cm carbon felt from Carbone Industrie, France). The specific surface per volume unit of about 42 cm2/cm3 has been estimated, as in Coche and Moutet (1987), by recording the electrochemical signal of reduction of an electroactive compound such as ferricyanide ion. The porosity of the felt used is about 95% (Gonzalez-Garcia et al. 1999).

The polypyrrole-biotin modified support was rinsed with distilled water and immersed in 2 ml of avidin aqueous solution (1 mg/ml) for 1 h. The resulting porous carbon membrane was washed with distilled water and immersed in 2 ml of biotinylated enzyme solution (2 mg/ml) for 1 h and then washed again with distilled water.

Fig. 24. Schematic diagram of the enzymatic membrane device. Glass column dimensions diameter 1 cm, height 10 cm. The inset represents the enzyme linkage of the biotinylated enzyme to the electropolymerized polypyrrole-biotin by the avidin-biotin molecular recognition process

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