Enzyme Immobilization by Molecular Recognition

Cation-exchange textiles are modified according to the following procedure: a sample of textile (4 cm2) is immersed in NaOH and urea solution (1M) for 12 h to graft amino groups on carboxylic acid functions. Biotin is attached to this amino group using N-hydroxysuccinimide biotin as a coupling reagent (0.4mg/ml for 12 h at room temperature). Anion-exchange textiles are modified directly by immersion in a sulfo-N-hydroxysuccinimide biotin (0.4mg/ml) aqueous solution for 12 h. All textiles are then thoroughly rinsed with distilled water and stored at room temperature before being used.

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