Experimental Protocols

Typical protocols start with a waveguide sensor chip that has been pre-functionalised with a covalently bound species such as free amine (coupled to the waveguide surface via silane chemistry) or free thiol (again covalently bound using silane chemistries). The chip is inserted into the instrument and the fluidic system is started (typically flowing running buffer at 100 ^l min-1) followed by a series of injections such as linker [e. g. homobifunctional linker such as bis(sulphosuccinimydyl) suberate, BS3, an amine-amine linker], followed by protein followed by block [such as Tris(hydroxymethyl)aminomethane, Tris]. The surface is then ready to be challenged as required by the experimentalist.

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