Protein Charge Heterogeneity

Based on electrostatics, can a positively (negatively) charged protein be attracted to a positively (negatively) charged surface? Within the Poisson-

Boltzmann approach, the answer is yes, according to Asthagiri and Lenhoff (1997), so long as both protein and surface contain charge heterogeneity. The idea is that regions of complementary charge may come into close contact, while overall repulsion is sufficiently screened by counterions so that a net attraction occurs. This explanation appears to most plausibly explain our observed increase in cytochrome c adsorption with increasing adsorbent potential.

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