Abbreviations

ACR

Acute cellular rejection

ALL

Acute lymphoblastic leukemia

ARMS

Amplification refractory

mutation system

ARP

Acidic ribosomal

phosphoprotein

ASO

Allele-specific oligonucleotide

ATP

Adenosine triphosphate

p2m

P2-microglobulin (B2M)

BC

Background control (in

immuno-PCR)

BHQ

Black hole quencher

BKV

BK virus

BLAST

Basic local alignment search

tool

BSA

Bovine serum albumin

CCD

Charge-coupled device

cDNA

Complementary DNA

ChIP

Chromatin

immunoprecipitation

CML

Chronic myeloid leukemia

CMV

Cytomegalovirus

COX

Cytochrome c oxidase

cp

Crossing point (LightCycler®

terminology)

CRS

Cambridge Reference Sequence

Ct

Threshold cycle (ABI

terminology)

CTAB

Cetyltrimethylammonium

Bromide

CV

Coefficient of variation

(standard deviation divided by

the mean)

CVS

Chorionic villi sampling

CYC

cyclophilin

DABCYL

4-(dimethylamino)azobenzene-

4'-carboxylic acid

DABSYL

4-(dimethylamino)azobenzene-

4'-sulfonyl chloride

DART

Data analysis for real-time PCR

DFA

Direct fluorescence assay

DGGE

Denaturing gradient gel

electrophoresis

ds

Double-stranded

dTTP

Deoxythymidine triphosphate

dUTP

2' deoxyurindine

5' triphosphate

EAC

Europe against cancer

EBV

Epstein-Barr virus

ELISA

Enzyme-linked immunosorbent

assay

FACS

Fluorescent-assisted cell

sorting

FAM

6-carboxy fluorescein

FDM

First derivative maximum

FISH

Fluorescence in-situ

hybridization

FITC

Fluorescein

FRET

Fluorescence resonance energy

transfer

GAPDH

glyceraldehyde-3-phosphate

dehydrogenase

gDNA

Genomic DNA

GOI

Gene of interest

GUS

P-glucuronidase

HBV

Hepatitis B virus

HCV

Hepatitis C virus

HEP

Human tpigenome Project

HEX

Hexachloro-carbonyl-

fluorescein

HKG

House keeping gene

HPLC

High-performance liquid

chromatography

HPRT

Hypoxanthine phosphoribosyl-

transferase 1

HSV

Herpes simplex virus

GOI

Gene of interest

IC

Internal control (normalizer,

reference)

IFN

Interferon

IL

Interleukin

IPC

Internal positive control

IVF

In-vitro fertilization

JOE

2, 7-dimethoxy-4, 5-dichloro-6-

carboxy-fluoroscein

KOD

Kinetic outlier detection

LATE PCR

Linear after the exponential PCR

r2

R-squared or coefficient of

LCM

Laser capture microdissection

determination (varies between 0

LED

Light emitting diode

and +1)

LiCl

Lithium Chloride

rCRS

Revised Cambridge Reference

LNA

Locked nucleic acid

Sequence

LUXâ„¢

Light upon extension

REST

Relative expression software

mC

Methylated cytosine

tool

MDR1

Multidrug resistance protein 1

REST-MCS

REST - multiple condition

MGB

Minor groove binders

solver

MHC

Major histocompatibility

REST-RG

REST Rotor-Gene

complex

RFLP

Restriction fragment length

MMF

Mycophenolate mofetil

polymorphism

MRD

Minimal residual disease

ARn

Normalized reporter signal

mRNA

Messenger RNA

minus background fluorescence

MSE

Mean squared error (nothing to

-RTC

Minus reverse transcriptase

do with SEM)

control

MSP

Methylation-specific PCR

Rn

Normalized raw fluorescence

mtDNA

Mitochondrial DNA (see also

ROS

Reactive oxygen species

nDNA)

ROX

6-carboxy-X-rhodamine

NAC

No amplification control

rRNA

Ribosomal RNA

NASBA

Nucleic acid sequence based

RSV

Respiratory syncytial virus

amplification

RT

Reverse transcription

nDNA

Nuclear DNA (as opposed to

SD

Standard deviation

mtDNA)

SDM

Second derivative maximum

NF

Normalization factor

SDS

Sequence Detection Software

NTC

No template control (no target

SEM

Standard error of the mean

control)

SMCC

Succinimidyl-4-(N-

OD

Optical density

maleimidomethyl) cyclohexane-

od26O

Optical density at 260 nm

l-carboxylate

PAGE

Polyacrylamide gel

SNP

Single nucleotide

electrophoresis

polymorphism

PCR

Polymerase chain reaction

STR

Short tandem repeat

PCV

Packed cell volume

TAMRA

6-carboxy-tetra-methyl-

PGD

Preimplantation genetic

rhodamine

diagnosis

TBP

TATA-binding protein

PGK

Phosphoglycerokinase

TCA

Trichoroacetic acid

PMT

Photomultiplier tube

TCR

T-cell receptor

PND

Prenatal diagnosis

TE buffer

Tris-EDTA buffer

PTLD

Post-transplant

TfR

Transferrin receptor

lymphoproliferative disorders

Tm

Melting temperature

qPCR

Quantitative PCR

TREC

T-cell receptor excision circles

r

(Pearson's) coefficient of

tRNA

Transfer RNA

correlation (varies between -1

UNG

Uracil-N-glycosylase

and +1)

y-int

y-intercept

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