A common problem facing researchers investigating gene expression is that of biological variance. Even when factors such as genetic background and environment may be controlled, biological variance will exist between individuals (Pritchard et al., 2001), resulting in noisy data and necessitating large sample sizes. Whilst many qPCR platforms enable 96-sample formats, many researchers utilize sample replicates, typically triplicates, to ensure each data point is well-resolved. The use of large samples may become problematic due to inter-assay variability, as data from samples split across different experimental runs is more difficult to directly compare.
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