This chapter outlines the many uses of SYBR® Green I-based real-time PCR. Experimental protocols using SYBR® Green in gene quantification are presented in Chapters 5 and 7. It appears that this inexpensive alternative to specific probe-based chemistries has overcome the early skepticism about its use. No doubt, developments in melting curve analysis have contributed to its popularity and even allowed multiplexing of allelic discrimination assays. SYBR® Green I or other recently discovered dsDNA binding dye-based real-time PCR techniques can be used in allelic discrimination or quantification assays and will most probably continue to gain popularity.

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