Determine exonexon boundaries

Use the human genome browser, click on the accession number for the human mRNA corresponding to the full-length transcript (e.g. X00351). Click on the accession number at the top to display the sequence database information. Click on the accession number under 'query' in the mRNA/Genomic alignment section to obtain 5' and 3' sequences (red), coding region (blue), ATG and STOP codons (underlined), exon-exon boundaries (bold, light blue or orange). Avoid inconsistent splicing sites and polyadenylation signals (underlined but not always).

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