Nase treatment

Briefly treat the RNA with RNase-free DNase to remove any residual genomic DNA that may be present in the RNA. Prepare the DNase master mix accounting for 1-2 additional reactions.

Ingredient Per reaction For x reactions

22. Add 1.2 pg of RNA, 4.5 pl of master mix and water to 30 pl into 200 pl PCR strip tubes.

23. Mix by gentle flicking, and briefly spin on a minicentrifuge that can handle strip tubes. Using a PCR Thermal Cycler, incubate at 37°C for 10 min and then 90°C for 5 min to inactivate the DNase.

Was this article helpful?

0 0

Post a comment