Identify the target sequence

Browse the human genome project database with P-actin as the keyword to identify the sequence, chromosome location (7p22.1), mRNA accession number (X00351), mRNA length (1761 bp) and gene structure (5 exons).

To choose a suitable accession number to base primer design on, use the graphic representation to select an mRNA that includes all the exons and has 5' and 3' sequence (the database is full of all sorts of partial cDNA sequences (e.g. CR685511) and mRNA with 'alternative' or 'inconsistent' exons (e.g. AK097861)).

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