Primer and probe design tools

• Primer3 (http://www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi). Primer3 is a popular free design tool for PCR primers and probes. Users can customize many PCR parameters.

• Primer Express® (http://www.appliedbiosystems.com). This is a commercial desktop program from Applied Biosystems. It is widely used for design of TaqMan primers and probes.

• Premier Biosoft International (http://www.premierbiosoft.com). This is a commercial web site for real-time PCR primer and probe design.

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