Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74: 5350-4.
Ausubel FM (2001) Current protocols in molecular biology. Wiley InterScience, New York.
Bustin SA (2004) A-Z of quantitative PCR. International University Line, La Jolla, CA.
Calogero A, Hospers GA, Timmer-Bosscha H, Koops HS, Mulder NH (2000) Effect of specific or random c-DNA priming on sensitivity of tyrosinase nested RT-PCR: potential clinical relevance. Anticancer Res 20: 3545-3548.
Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15: 532-534, 536-537.
Didenko VV (2001) DNA probes using fluorescence resonance energy transfer (FRET): designs and applications. Biotechniques 31: 1106-1116, 1118, 1120-1121.
Gerard GF, Fox DK, Nathan M, D'Alessio JM (1997) Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA. Mol Biotechnol 8: 61-77.
Gjerdrum LM, Sorensen BS, Kjeldsen E, Sorensen FB, Nexo E, Hamilton-Dutoit S (2004) Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis. J Mol Diagn 6: 42-51.
Goldenberg O, Landt O, Schumann RR, Gobel UB, Hamann L (2005) Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCR. Biotechniques 38: 29-30, 32.
Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6: 986-994.
Lakowicz JR (1983) Principles of fluorescence spectroscopy. Plenum Press, New York.
Liao Z, Chen M, Guo L, Gong Y, Tang F, Sun X, Tang K (2004) Rapid isolation of high-quality total RNA from taxus and ginkgo. Prep Biochem Biotechnol 34: 209-214.
Mullis KB (1990) Target amplification for DNA analysis by the polymerase chain reaction. Ann Biol Clin (Paris) 48: 579-582.
Peters IR, Helps CR, Hall EJ, Day MJ (2004) Real-time RT-PCR: considerations for efficient and sensitive assay design. J Immunol Methods 286: 203-217.
Pierce KE, Sanchez JA, Rice JE, Wangh LJ (2005) Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection. Proc Natl Acad Sci USA 102: 8609-8614.
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Sanchez JA, Pierce KE, Rice JE, Wangh LJ (2004) Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative realtime analysis. Proc Natl Acad Sci USA 101: 1933-1938.
Selvin PR (1995) Fluorescence resonance energy transfer. Methods Enzymol 246: 300-334.
Sherrill CB, Marshall DJ, Moser MJ, Larsen CA, Daude-Snow L, Prudent JR (2004) Nucleic acid analysis using an expanded genetic alphabet to quench fluorescence. J Am Chem Soc 126: 4550-4556.
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503-517.
Vu HL, Troubetzkoy S, Nguyen HH, Russell MW, Mestecky J (2000) A method for quantification of absolute amounts of nucleic acids by (RT)-PCR and a new mathematical model for data analysis. Nucleic Acids Res 28: E18.
Winer J, Jung CK, Shackel I, Williams PM (1999) Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem 270: 41-49.
Was this article helpful?