The time course of interaction between Y941 and SH2n observed with sRL91 is shown in Fig. 4.4a. The luminescence intensities increased within 5 min after insulin stimulation and gradually decreased afterward. This time dependence of the interaction is due to tyrosine phosphorylation and dephosphorylation, which is in good agreement with the immunoblot analysis of anti-phosphotyrosine antibody shown in Fig. 4.4b. The results indicate that the luminescence activity of sRL91 directly reflects the ongoing PPI in living cells.
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